D1-4G2-4-15細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
ATCC Number： HB-112?
細(xì)胞形態(tài)： **樣
**類型： IgG2a and IgG1
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
生長狀態(tài)： 懸浮生長
Designations: D1-4G2-4-15
Depositors: MK Gentry
D1-4G2-4-15細(xì)胞Isotype: IgG2a and IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; antibody against flavivirus group antigen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: Tested and found negative for ectromelia virus (mousepox).
The antibody reacts with flavivirus group specific antigens.
Comments: D1-4G2-4-15細(xì)胞The antibody reacts with flavivirus group specific antigens.
Tested and found negative for ectromelia virus (mousepox).
Animals were immunized with dengue virus type 2 antigens.
Spleen cells were fused with P3X63Ag8 myeloma cells.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Temperature: 37.0°C
Subculturing: D1-4G2-4-15細(xì)胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 922: Henchal EA, et al. Dengue virus-specific and Flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am. J. Trop. Med. Hyg. 31: 830-836, 1982. D1-4G2-4-15細(xì)胞PubMed: 6285749
