BT細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 牛

品系： Holstein

ATCC Number： CRL-1390?

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來(lái)源： 其他

運(yùn)輸方式： 凍存運(yùn)輸

年限： newborn, calf

數(shù)量： 大量

BT細(xì)胞Designations: BT

Depositors: AW McClurkin

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Bos taurus

Morphology:

Source: Organ: turbinate

Strain: Holstein

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Age: BT細(xì)胞newborn, calf

Comments: This line has been tested, and found negative for, Bovine viral diarrhea virus (BVDV). The cells should not be grown in medium containing serum of bovine origin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: culture medium 95%; DMSO, 5%

Storage temperature: BT細(xì)胞liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2040

References: 23135: McClurkin AW, et al. Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus. Arch. Virusforsch. 45: 285-289, 1974. PubMed: 4472511

28594: Bermudez-Cruz R, et al. Characterization of an abundant, unique 1.7-kilobase bovine herpesvirus 4 (BHV-4) late RNA and mapping of a BHV-4 IE2 transactivator-binding site in its promoter-regulatory region. J. Virol. 71: 527-538, 1997. BT細(xì)胞PubMed: 8985381