WPE1-NB26-65細(xì)胞

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 其他

細(xì)胞類型： 其他細(xì)胞類型

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

ATCC Number： CRL-2890?

數(shù)量： 大量

年限： 54

Designations: WPE1-NB26-65

Depositors: MM Webber

WPE1-NB26-65細(xì)胞Biosafety Level: 2 [cells containing human HPV-18 viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: prostate peripheral zone

Cell Type: epithelialHPV-18 transfected

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1994

Receptors: androgen receptor, expressed (upregulated upon exposure to androgen)

Tumorigenic: YES

Antigen Expression: kallikrein 3 ( KLK3); prostate specific antigen (PSA); upon exposure to androgen

DNA Profile (STR): Amelogenin: X,Y

WPE1-NB26-65細(xì)胞CSF1PO: 13

D13S317: 8,14

D16S539: 9,11

D5S818: 12,15

D7S820: 10,11

THO1: 8

TPOX: 8,11

vWA: 14,18

Age: 54

Gender: male

Ethnicity: Caucasian

Comments: WPE1-NB26-65 and WPE1-NB26-64 (ATCC CRL-2889) were derived from a subcutaneous tumor in a nude mouse injected with WPE1-NB26 (ATCC CRL-2852). WPE1-NB26-65細(xì)胞They secrete matrix metalloproteinases 9 and 2 (MMP-9 and MMP-2) into the culture medium while the parent line produces barely detectable levels. WPE1-NB26-65 secretes 40% higher levels of MMP-2 than WPE1-NB26-64. [PubMed: 16471037]WPE1-NB26 cells belong to a family of cell lines, referred to as the MNU cell lines, which are all derived from RWPE-1 (ATCC CRL-11609) cells after exposure to MNU. The MNU cell lines, in order of increasing malignancy are: WPE1-NA22 (ATCC CRL-2849), WPE1-NB14 (ATCC CRL-2850), WPE1-NB11 (ATCC CRL-2851) and WPE1-NB26 (ATCC CRL-2852). The WPE1-NB26-64 and WPE1-NB26-65 cell lines show an increase in anchorage-dependent growth and invasive ability as compared to the parent WPE1-NB26.

The depositor reports that the parent RWPE-1 cell line was screened for Hepatitis B (HBV, Hepatitis C (HCV) and human immunodeficiency (HIV) viruses and was found to be negative.

Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:

0.05 mg/ml BPE - provided with the K-SFM kit

5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C

Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.

Subculturing: Protocol: Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

WPE1-NB26-65細(xì)胞Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS).

Add 3.0 to 4.0 ml of 0.05% Trypsin - 0.53mM EDTA solution, diluted 1:1 with D-PBS, and place flask in a 37°C incubator for 5 to 8 minutes. Observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach.

Add 6.0 to 8.0 ml of 0.1% Soybean Trypsin Inhibitor (or 2% fetal bovine serum in D-PBS), as appropriate, and aspirate cells by gently pipetting.

Transfer cell suspension to centrifuge tube and spin at approximately 125 x g for 5 to 7 minutes.

Discard supernatant and resuspend cells in fresh serum-free growth medium. Add appropriate aliquots of cell suspension to new culture vessels. An inoculum of 6 X 10(3) to 7 X 10(3) viable cells/sq. cm is recommended.

Incubate cultures at 37°C. We recommend that you maintain cultures at a cell concentration between 4 X 10(4) and 6 X 10(4) cells/sq. cm.

Cells grown under serum-free or reduced serum conditions may not attach strongly during the 24 hours after subculture and should be disturbed as little as possible during that period.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended

Medium Renewal: Every 48 hours

Preservation: Freeze medium: Complete growth medium described above supplemented with 15% fetal bovine serum and 10% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Storage temperature: liquid nitrogen vapor phase

Doubling Time: about 23 hours

Related Products:WPE1-NB26-65細(xì)胞 recommended serum:ATCC 30-2020

Erythrosin B vital stain solution:ATCC 30-2404

Cell culture tested DMSO:ATCC 4-X

phosphate-buffered saline:ATCC 30-2200

parental cell line:ATCC CRL-11609

derived from same individual:ATCC CRL-11610

derived from same individual:ATCC CRL-2849

derived from same individual:ATCC CRL-2850

derived from same individual:ATCC CRL-2852

derived from same individual:ATCC CRL-2851

derived from same individual:ATCC CRL-2853

derived from same individual:ATCC CRL-2854

derived from same individual:ATCC CRL-2887

derived from same individual:ATCC CRL-2888

derived from same individual:ATCC CRL-2889

derived from an unrelated individual:ATCC CRL-11611

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46795: Webber MM, et al. Acinar differentiation by non-malignant immortalized human prostatic epithelial cells and its loss by malignant cells. Carcinogenesis 18: 1225-1231, 1997. PubMed: 9214606

46799: Webber MM, et al. Prostate specific antigen and androgen receptor induction and characterization of an immortalized ***** human prostatic epithelial cell line. Carcinogenesis 17: 1641-1646, 1996. PubMed: 8761420

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53180: Webber MM, et al. Immortalized and tumorigenic ***** human prostatic epithelial cell lines: characteristics and applications. Part I. Cell markers and immortalized nontumorigenic cell lines. Prostate 29: 386-394, 1996. PubMed: 8977636

53181: Webber MM, et al. Immortalized and tumorigenic ***** human prostatic epithelial cell lines: characteristics and applications Part 2. Tumorigenic cell lines. Prostate 30: 58-64, 1997. PubMed: 9018337

53182: Webber MM, et al. Immortalized and tumorigenic ***** human prostatic epithelial cell lines: characteristics and applications. Part 3. Oncogenes, suppressor genes, and applications. Prostate 30: 136-142, 1997. PubMed: 9051152

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53193: Achanzar WE, et al. Cadmium induces c-myc, p53, and c-jun expression in normal human prostate epithelial cells as a prelude to apoptosis. Toxicol. Appl. Pharmacol. 164: 291-300, 2000. PubMed: 10799339

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89996: Quader ST, et al. Evaluation of the chemopreventive potential of retinoids using a novel in vitro human prostate carcinogenesis model. Mutat. Res. 496: 153-161, 2001. PubMed: 11551491

89997: Webber MM, et al. A human prostatic stromal myofibroblast cell line WPMY-1: a model for stromal-epithelial interactions in prostatic neoplasia. Carcinogenesis 20: 1185-1192, 1999. PubMed: 10383888

89998: Bello-DeOcampo D, et al. The role of alpha 6 beta 1 integrin and EGF in normal and malignant acinar morphogenesis of human prostatic epithelial cells. Mutat. Res. 480-481: 209-217, 2001. PubMed: 11506815

90372: Webber MM, et al. Modulation of the malignant phenotype of human prostate cancer cells by N-(4-hydroxyphenyl)retinamide (4-HPR). Clin. Exp. Metastasis 17: 255-263, 1999. PubMed: 10432011

90375: Sharp RM, et al. N-(4-hydroxyphenyl)retinamide (4-HPR) decreases neoplastic properties of human prostate cells: an agent for prevention. Mutat. Res. 496: 163-170, 2001. PubMed: 11551492

90376: Carruba G, et al. Regulation of cell-to-cell communication in non-tumorigenic and malignant human prostate epithelial cells. Prostate 50: 73-82, 2002. PubMed: 11816015

90377: Achanzar WE, et al. Altered apoptotic gene expression and acquired apoptotic resistance in cadmium-transformed human prostate epithelial cells. Prostate 52: 236-244, 2002. PubMed: 12111698

90378: Carruba G, et al. Intercellular communication and human prostate carcinogenesis. Ann. N.Y. Acad. Sci. 963: 156-168, 2002. PubMed: 12095941

90379: Saladino F, et al. Connexin expression in nonneoplastic human prostate epithelial cells. Ann. N.Y. Acad. Sci. 963: 213-217, 2002. PubMed: 12095946

90380: Hegarty PK, et al. Effects of cyclic stretch on prostatic cells in culture. J. Urol. 168: 2291-2295, 2002. PubMed: 12394777

90383: Lugassy C, et al. Human melanoma cell migration along capillary-like structures in vitro: a new dynamic model for studying extravascular migratory metastasis. J. Invest. Dermatol. 119: 703-704, 2002. PubMed: 12230517

90384: Brambila EM, et al. Chronic arsenic-exposed human prostate epithelial cells exhibit stable arsenic tolerance: mechanistic implications of altered cellular glutathione and glutathione S-transferase. Toxicol. Appl. Pharmacol. 183: 99-107, 2002. PubMed: 12387749

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92798: Rivette AS, et al. Selection of cell lines with enhanced invasive phenotype from xenografts of the human prostate cancer cell line WPE1-NB26. J. Exp. Ther. Oncol. 5: 111-123, 2005. PubMed: 16471037