HT-2 clone A5E細胞

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 小鼠

器官來源： 脾

細胞形態(tài)： **樣

品系： BALB/c

ATCC Number： CRL-1841?

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

數(shù)量： 大量

Designations: HT-2 clone A5E

Depositors: JF Weaver, A Creasey

HT-2 clone A5E細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: spleen

Strain: BALB/c

Cell Type: IL-2 dependent T lymphocyte;

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Receptors: interleukin 2 (IL-2)

HT-2 clone A5E細胞Antigen Expression: Thy-1

Comments: The HT-2 cell line was derived in 1979 by J. Watson at the University of Auckland from a culture of BALB/c spleen cells activated by sheep erythrocytes in the presence of IL-2.

The A5E clone was selected to provide a homogeneous population that is highly sensitive to IL-2.

The line is dependent on IL-2 for growth and can be used to measure IL-2 activity in a very rapid and sensitive assay.

The cells are commonly used for characterization of lymphokines and cytokines.

In addition to IL-2, substantial proliferation is seen in the presence of interleukin 4 (interleukin-4, IL-4, also known as BSF-1).

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 100-200 IU/ml IL-2, 90%; fetal bovine serum, 10%Alternatively, IL-2 in the form of Rat T-STIM factor with Con A (available from Becton Dickinson, Cat.# 354115) may be used at 10% by volume.

Temperature: 37.0°C

Subculturing: HT-2 clone A5E細胞Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Subcuture every two days at 3.5 X 10 exp4 viable cells/ml.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1763: Kupper T, et al. Growth of an interleukin-2/interleukin 4-dependent T cell line induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). J. Immunol. 138: 4288-4292, 1987. PubMed: 2953804

1764: Ho SN, et al. Differential bioassay of interleukin-2 and interleukin-4. J. Immunol. Methods 98: 99-104, 1987. PubMed: 3494085

4396: Lee F, et al. Isolation and characterization of a mouse interleukin cDNa clone that expresses B-cell stimulatory factor 1 activities and T-cell and mast-cell-stimulating activities. Proc. Natl. Acad. Sci. USA 83: 2061-2065, 1986. PubMed: 3083412

22359: Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J. Exp. Med. 150: 1510-1519, 1979. PubMed: 92524

22459: . . J. Biol. Response Modif. 4: 96-109, 1985.

23462: Kupper T, et al. Autocrine growth of T cells independent of interleukin 2: identification of interleukin 4 (IL 4, BSF-1) as an autocrine growth factor for a cloned antigen-specific helper T cell. J. Immunol. 138: 4280-4287, 1987. PubMed: 2953803

23463: Woods A, et al. Granulocyte-macrophage colony stimulating factor produced by cloned L3T4a+, class II-restricted T cells induces HT-2 cells to proliferate. J. Immunol. 138: 4293-4297, 1987. HT-2 clone A5E細胞PubMed: 2953805

33122: Kotanides H, Reich NC. Interleukin-4-induced STAT6 recognizes and activates a target site in the promoter of the interleukin-4 receptor gene. J. Biol. Chem. 271: 25555-25561, 1996. PubMed: 8810328