LADMAC細(xì)胞

器官來源： 骨髓

細(xì)胞形態(tài)： **樣

ATCC Number： CRL-2420?

數(shù)量： 大量

年限： *****

生長狀態(tài)： 懸浮生長，少量貼壁

品系： C3H

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

Designations: LADMAC

LADMAC細(xì)胞Depositors: WS Walker

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension, with some loosely adherent cells

Organism: Mus musculus deposited as mouse

Morphology: lymphoblast

Source: Organ: bone marrow

Strain: C3H

Cellular Products: colony stimulating factor-1 (CSF-1)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Age: *****

Comments:LADMAC細(xì)胞 LADMAC is a transformed cell line derived by transfecting mouse bone marrow cells highly enriched for macrophage progenitors with cloned human cellular myc-homologous sequences covalently attached to pBR325 (pR myc).

The cell line has monocyte-like morphology; contains nonspecific esterase; is phagocytic for latex beads; secretes lysozyme, and bears the Mac-1 antigen.

A minority of cells are Fc receptor positive and an appreciable number of cells are complement receptor 1 positive.

The cells are tumorigenic in nu+, nu+ mice but not in syngenic mice. The cells are not phagocytic for antibody or complement-coated particles; they do not constitutively secrete Interleukin-1.

LADMAC cells secrete the growth factor colony stimulating factor 1 (CSF-1). CSF-1 is capable of supporting the in vitro proliferation of mouse bone marrow macrophages. [38883]

The Pannell-Milstein roller bottle apparatus may be used to produce high concentrations of CSF-1. [38884]

This cell line is used to produce LADMAC conditioned medium. It will support the growth of the macrophage cell lines EOC 2 (CRL-2467), EOC 13.31 (CRL-2468), EOC 20 (CRL-2469), I-11.15 (CRL-2470) and I-13.35 (CRL-2471).

Propagation: ATCC complete growth medium: LADMAC細(xì)胞The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Attached cells may be subcultured by tapping the sides of the flask until cells are dispersed.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 38883: Sklar MD, et al. LADMAC細(xì)胞Transformation of mouse bone marrow cells by transfection with a human oncogene related to c-myc is associated with the endogenous production of macrophage colony stimulating factor 1. J. Cell. Physiol. 125: 403-412, 1985. PubMed: 3877730

38884: Olivas E, et al. Use of the Pannell-Milstein roller bottle apparatus to produce high concentrations of the CSF-1, the mouse macrophage growth factor. J. Immunol. Methods 182: 73-79, 1995. PubMed: 7769247