Pfeiffer細(xì)胞
年限： ***** *****
生長狀態(tài)： 懸浮生長
ATCC Number： CRL-2632?
相關(guān)**： 其他**
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞類型： 其他細(xì)胞類型
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
是否是腫瘤細(xì)胞： 0
物種來源： 人
**類型： IgG kappa
Designations: Pfeiffer
Depositors: H Ben-Bassat
Pfeiffer細(xì)胞Isotype: IgG kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens deposited as human
Morphology: lymphoblast
Source: Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Derived from metastatic site: pleural effusion
Cell Type: lymphoblast B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1992
Applications: Pfeiffer細(xì)胞The cells are negative for Epstein-Barr virus.
The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei.
Antigen Expression: CD10; Homo sapiens
CD19; Homo sapiens
CD20; Homo sapiens
CD38; Homo sapiens
CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]
DNA Profile (STR): CSF1PO: 10, 11
D13S317: 11, 12
D16S539: 12
D5S818: 10, 13
D7S820: 8, 12
THO1: 9, 9.3
TPOX: 9
vWA: 17, 18
Amelogenin: XY
Cytogenetic Analysis: The cell line contains several chromosomal abberations including the typical t(14;18)(q32;q21) translocation of follicular lymphomas.
Pfeiffer細(xì)胞Age: ***** *****
Gender: male
Ethnicity: White
Comments: The Pfeiffer cell line was established in 1992 from a patient in the leukemic phase of diffuse large cell lymphoma (DLCL) with cleaved and non-cleaved nuclei.
The cells are negative for Epstein-Barr virus.
This cell line is a model system for studying non-Hodgkin lymphomas.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Doubling Time: 24 to 30 hrs
Related Products: Pfeiffer細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53289: Gabay C, et al. Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
