DEC-205 [NLDC-145]細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 大鼠

ATCC Number： HB-290?

細(xì)胞形態(tài)： **樣

**類型： IgG2a

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: DEC-205 [NLDC-145]

Depositors: RM Steinman, G Kraal

Isotype: IgG2a

DEC-205 [NLDC-145]細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: DEC-205 [NLDC-145]細(xì)胞Animals were immunized with mouse lymph node stroma.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with an antigen that is expressed at high levels on mouse dendritic cells and thymic cortical epithelial cells.

The antigen is a 205,000 dalton group VI C type molecule that mediates adsorptive endocytosis and antigen presentation.

The antibody reacts with and stains mouse dendritic cells and epidermal Langerhans cells.

The cells are capable of being adapted to serum free growth in using Nutridoma medium.

Propagation: DEC-205 [NLDC-145]細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22819: Kraal G, et al. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J. Exp. Med. 163: 981-997, 1986. DEC-205 [NLDC-145]細(xì)胞PubMed: 3950549