RAW 264.7 gamma NO(-)細(xì)胞

品系： BALB/c

運輸方式： 凍存運輸

生長狀態(tài)： 貼壁生長

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： CRL-2278?

數(shù)量： 大量

Designations: RAW 264.7 gamma NO(-)

Depositors: SW Russell

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

RAW 264.7 gamma NO(-)細(xì)胞Organism: Mus musculus

Morphology:

Source: Strain: BALB/c

Cellular Products: lysozyme

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Receptors: complement (C3), expressed

Antigen Expression: H-2d

Gender: male

Comments: RAW 264.7 gamma NO(-) was derived from the RAW 264.7 (see ATCC TIB-71) mouse monocyte/macrophage cell line ordinally obtained in 1978 from Dr. Peter Ralph.

The cells were not intentionally cloned but were serendipitously obtained during routine culture.

Unlike the parental line, RAW 264.7 gamma NO(-) does not produce nitric oxide upon treatment with interferon gamma alone, but requires LPS for full activation (the iNOS promoter linked to a luciferase reporter gene is also unresponsive to IFN- alone).

This property makes its behavior more like that of normal macrophages from some commonly used strains of mice (e.g. C3H/HeN).

Propagation: RAW 264.7 gamma NO(-)細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cells can be grown as a monolayer or in spinner cultures. For routine passage cells should be grown on bacterial grade culture dishes. Subcultures are prepared by scraping cells from floor of dishes every two days and diluting to 1 x 10(6) cells/20ml (3 x 10(5) cells/20ml for weekend passage). Cells grown in spinner flasks are inoculated at a density of 1 x 10(5) cells/ml. Spinner culture cell densities should not be allowed to rise above 6 x 10(5) cells/ml.

RAW 264.7 gamma NO(-)細(xì)胞Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

parental cell line:ATCC TIB-71

References: 1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. RAW 264.7 gamma NO(-)細(xì)胞Immunol. 119: 950-954, 1977. PubMed: 894031

1207: Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198

22778: Alley EW, et al. A classical enhancer element responsive to both lipopolysaccharide and interferon-gamma augments induction of the iNOS gene in mouse macrophages. Gene 158: 247-251, 1995. PubMed: 7541763

23410: Lorsbach RB, et al. Expression of the nitric oxide synthase gene in mouse macrophages activated for tumor cell killing. Molecular basis for the synergy between interferon-gamma and lipopolysaccharide. J. Biol. Chem. 268: 1908-1912, 1993. PubMed: 7678412