SVR bag4細胞
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
組織來源： islet of Langerhans; endothelium
ATCC Number： CRL-2387?
細胞類型： SV40轉(zhuǎn)化細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
器官來源： 胰腺
Designations: SVR bag4
Depositors: JL Arbiser
SVR bag4細胞Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology:
Source: Organ: pancreas
Tissue: islet of Langerhans; endothelium
Cell Type: endothelialSV40 transformed
Permits/Forms: SVR bag4細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: SVR bag4 is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280) by infecting SVR cells with a retrovirus encoding beta-galactosidase and puromycin resistance.
SVR is a hygromycin resistant mouse endothelial cell line containing a temperature sensitive SV40 large T-antigen and H-RAS oncogene.
SVR bag4 can be used as a wild-type control for SVR A221a (ATCC CRL-2386). SVR bag4細胞It can be used to determine if drugs regulate the MAPKK pathway.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 1.0 g/L glucose, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 51400: LaMontagne KR Jr., et al. SVR bag4細胞Inhibition of MAP kinase kinase causes morphological reversion and dissociation between soft agar growth and in vivo tumorigenesis in angiosarcoma cells. Am. J. Pathol. 157: 1937-1945, 2000. PubMed: 11106566
