MDA-MB-134-VI細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

ATCC Number： HTB-23?

相關(guān)**： 導(dǎo)管癌

組織來(lái)源： duct

數(shù)量： 大量

年限： 47 years *****

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 單層生長(zhǎng)

器官來(lái)源： **

運(yùn)輸方式： 凍存運(yùn)輸

Designations: MDA-MB-134-VI

Depositors: R Cailleau

MDA-MB-134-VI細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: monolayer (lightly adherent)

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: mammary gland; breast

Tissue: duct

Disease: ductal carcinoma

Derived from metastatic site: pleural effusion

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Receptors: fibroblast growth factor (FGF)

Antigen Expression: Blood Type O; Rh+

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 8

D16S539: 13

D5S818: 11,12

D7S820: 10,11

THO1: 6,9

MDA-MB-134-VI細(xì)胞TPOX: 8,11

vWA: 14,15

Cytogenetic Analysis: hypodiploid; modal number = 43 with an extra long chromosome 2-like marker; (P28/29) hypodiploid and hypertriploid to hypotetraploid with large subtelocentric marker

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 1

PGM1, 1

PGM3, 1

Age: 47 years *****

Gender: female

Ethnicity: Caucasian

Comments: This is one of a series of breast tumor lines (ATCC HTB-23 through 26) isolated from pleural effusions and characterized by R. Cailleau and associates in 1973.

The cells grow a monolayer of round loosely adherent cells.

They grow slowly, becoming heavy (shedding somewhat into the medium) without actually becoming confluent.

The cells overexpress FGF receptors, and the fgfr-1 gene is amplified.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol: MDA-MB-134-VI細(xì)胞Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: lquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337

22182: Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.

22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779

22442: McLeskey SW, et al. MDA-MB-134 breast carcinoma cells overexpress fibroblast growth factor (FGF) receptors and are growth-inhibited by FGF ligands. Cancer Res. 54: 523-530, 1994. PubMed: 7506125

22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202

23093: Faust JB, Meeker TC. MDA-MB-134-VI細(xì)胞Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

26319: Cruciger QV, et al. Human breast carcinomas: marker chromosomes involving 1q in seven cases. Cytogenet. Cell Genet. 17: 231-235, 1976. PubMed: 1001030