OK細胞

生長狀態(tài)： 貼壁生長

數量： 大量

運輸方式： 凍存運輸

ATCC Number： CRL-1840?

相關**： 正常

細胞類型： 上皮細胞

是否是腫瘤細胞： 0

物種來源： 其他

細胞形態(tài)： 上皮樣

組織來源： proximal tubule

年限： *****

器官來源： 腎皮質

Designations: OK

OK細胞Depositors: MM Miller

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Didelphis marsupialis virginiana

Morphology: epithelial

Source: Organ: kidney, cortex

Tissue: proximal tubule

Disease: normal

Cell Type: epithelial

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Receptors: OK細胞alpha 2 adrenergic, expressed

atrial natriuretic peptide (ANP), expressed

serotonin, expressed

parathyroid hormone (PTH), expressed

alpha 2 adrenergic; serotonin; parathyroid hormone (PTH); atrial natriuretic peptide (ANP)

Age: *****

Gender: female

Comments: This line was originally developed for use as a source of X chromosomes in a study of X inactivation, and was subsequently found to be an excellent cell culture model for the kidney proximal tubule epithelium.

The cells display a variety of receptors in culture, and have been widely used as a model for studying those receptors.

Propagation: OK細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: OK細胞A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 1599: Yagil C, et al. Insulin binding, internalization, and degradation by a cultured kidney cell line. Am. J. Physiol. 254: E601-E608, 1988. PubMed: 2834959

1601: Cole JA, et al. Regulation of sodium-dependent phosphate transport by parathyroid hormone in opossum kidney cells: adenosine 3', 5'-monophosphate-dependent and -independent mechanisms. Endocrinology 122: 2981-2989, 1988. PubMed: 2836179

1602: Nakai M, et al. Atrial natriuretic factor inhibits phosphate uptake in opossum kidney cells as a model of renal proximal tubules. Biochem. Biophys. Res. Commun. 152: 1416-1420, 1988. PubMed: 2837187

22318: Cheng L, et al. Alpha-2-adrenergic modulation of the parathyroid hormone-inhibition of phosphate uptake in cultured renal (OK) cells. Biochem. Biophys. Res. Commun. 155: 74-82, 1988. PubMed: 2843189

22651: Koyama H, et al. Establishment and characterization of a cell line from the American opossum (Didelphys virginiana). In Vitro 14: 239-246, 1978. PubMed: 566717

23383: . . J. Pharmacol. 244: 571-578, 1988.