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產(chǎn)品資料

VLN6H2細胞

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產(chǎn)品名稱: VLN6H2細胞
產(chǎn)品型號: VLN6H2
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

VLN6H2細胞應(yīng)如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。VLN6H2細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


VLN6H2細胞  的詳細介紹

VLN6H2細胞


生長狀態(tài): 懸浮生長

數(shù)量: 大量

是否是腫瘤細胞: 0

物種來源: 人

運輸方式: 凍存運輸

細胞形態(tài): **樣

**類型: IgG

ATCC Number: HB-8633?

Designations: VLN6H2

Depositors: Regents of the University of California

Isotype: IgG

Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS ]

Shipped: frozen

VLN6H2細胞Medium & Serum: See Propagation

Growth Properties: suspension

Organism: human (B cell); human (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against cancer of the vulva

Permits/Forms: VLN6H2細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The fusion line, CRL-8061, is reported to be EBNA positive.

Lymphocytes were isolated from a regional draining lymph node of a patient with carcinoma of the vulva.

B lymphocytes were fused with UC 729-6 cells (see ATCC CRL-8061).

VLN6H2細胞The antibody recognizes an epitope on a 78000 dalton antigen on A-431 cells and a 66000 dalton antigen on T293H cells.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 8 X 10 exp5 cells/ml.

References: 21921: Glassy MC, Surh C. VLN6H2細胞Human-human hybrid cell lines that produce antibodies against antigenic determinants on cancer cells. US Patent 4,761,377 dated Aug 2 1988


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