C1498細(xì)胞
細(xì)胞形態(tài)： **樣
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
數(shù)量： 大量
ATCC Number： TIB-49?
相關(guān)**： 其他**
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
品系： C57BL/6J
運(yùn)輸方式： 凍存運(yùn)輸
Designations: C1498
Depositors: P Ralph
C1498細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Disease: acute myeloid leukemia
Strain: C57BL/6J
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. C1498細(xì)胞Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: The cells do not express B7-1.
Gender: female
Comments: They are resistant to 0.1 mM cortisol.
Tested and found negative for ectromelia virus (mousepox).
Propagation:C1498細(xì)胞 ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can beestablished by centrifugation with subsequent resuspension in fresh medium at 1 to 3 x 10 exp5 viable cells/ml. A saturation density of 1 to 2 X 10 exp6 is obtainable.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 92.5%; DMSO, 7.5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1104: Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304
22528: C1498細(xì)胞Ralph P, Nakoinz I. Inhibitory effects of lectins and lymphocyte mitogens on murine lymphomas and myelomas. J. Natl. Cancer Inst. 51: 883-890, 1973. PubMed: 4542714
30399: Boyer MW, et al. Thr role of B7 costimulation by murine acute myeloid leukemia in the generation and function of a CD8+ T-cell line with potent in vivo graft-versus-leukemia properties. Blood 89: 3477-3485, 1997. PubMed: 9129056
