SC-1細胞

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

器官來源： 胚胎

ATCC Number： CRL-1404?

是否是腫瘤細胞： 0

物種來源： 小鼠

年限： embryo

細胞形態(tài)： 成纖維樣

運輸方式： 凍存運輸

Designations: SC-1

SC-1細胞Depositors: JW Hartley

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Organ: embryo

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. SC-1細胞Please click here for information regarding the specific requirements for shipment to your location.

Age: embryo

Comments: Initial cultures from a thawed ampule may take 10 to 12 days to become confluent.

Tested and found negative for ectromelia virus (mousepox).

Propagation: SC-1細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 1 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.

References: 22696: Hartley JW, Rowe WP. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology 65: 128-134, 1975. SC-1細胞PubMed: 167514