4D1.5.7細胞
ATCC Number： HB-11495?
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
細胞形態(tài)： **樣
是否是腫瘤細胞： 0
物種來源： 小鼠
**類型： IgG2a
Designations: 4D1.5.7
Depositors: Genentech, Inc.
Isotype: IgG2a
4D1.5.7細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against interleukin 8 receptor type B (IL-8R-B, CDw128)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: 4D1.5.7細胞Animals were immunized with the 293-27 cell line (human fetal kidney, transfected to express IL-8 receptor type B).
Spleen cells were fused with P3X63Ag8U.1 myeloma cells.
The antibody binds to IL-8R-B with Kd = 1.9 nM.
It does not bind to the type A receptor.
The epitope recognized by 4D1.5.7 appears to be expressed in the N terminal portion of the molecule.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose and 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. 4D1.5.7細胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22079: Chuntharapai A, et al. Antibodies to human IL-8 type B receptor. US Patent 5,440,021 dated Aug 8 1995
23489: Chuntharapai A, et al. Monoclonal antibodies detect different distribution patterns of IL-8 receptor A and IL-8 receptor B on human peripheral blood leukocytes. J. Immunol. 153: 5682-5688, 1994. PubMed: 7527448
23490: 4D1.5.7細胞Chuntharapai A, Kim KJ. Regulation of the expression of IL-8 receptor A/B by IL-8: possible functions of each receptor. J. Immunol. 155: 2587-2594, 1995. PubMed: 7650389
