B27M2細(xì)胞

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： IgM

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： HB-165?

Designations: B27M2

Depositors: F Grumet

Isotype: IgM

Biosafety Level: 1

B27M2細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

B27M2細(xì)胞

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against HLA B27, Bw47; cytotoxic

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Comments: Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,A28,B27,B44,Bw4,Cw2).

Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.

The antibody is cytotoxic for a subpopulation of HLA B27 positive cells and for all HLA Bw47 positive cells at an appropriate titer.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Modified RPMI 1640 medium (see fact sheet), 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1171: B27M2細(xì)胞Grumet FC, et al. Monoclonal antibody (B27M2) subdividing HLA-B27. Hum. Immunol. 5: 61-72, 1982. PubMed: 6981637