B27M2細(xì)胞細(xì)胞
細(xì)胞形態(tài)： 上皮樣
品系： C3H/Mai
生長狀態(tài)： 貼壁生長
年限： weanling
細(xì)胞類型： SV40轉(zhuǎn)化細(xì)胞
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
ATCC Number： CCL-139?
B27M2細(xì)胞細(xì)胞器官來源： 腎臟
相關(guān)**： 正常
Designations: TCMK-1
Depositors: PH Black, W Rowe
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial
Source: Strain: C3H/Mai
Organ: kidney
Disease: normal
Cell Type: B27M2細(xì)胞細(xì)胞SV40 transformed
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Isolation: Isolation date: 1963
Virus Susceptibility: Vesicular stomatitis, Glasgow (Indiana)
Human poliovirus 1
Virus Resistance: poliovirus 1
Tumorigenic: No
Antigen Expression: H-2k
Cytogenetic Analysis: modal number = 78; range = 64 to 157.
All cells showed 2-6 acrocentric chromosomes with secondary constrictions and 1-4 biarmed chromosomes per cells. Sixty percent of the cells had one or more minute chromosomes.
Age: weanling
Comments: The cells are SV40 transformed and are positive for SV40 T-antigen.
About 2% of the cells are positive for SV40 viral antigen, but virus is not recoverable.
Tested and found negative for ectromelia virus (mousepox).
Propagation: B27M2細(xì)胞細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: B27M2細(xì)胞細(xì)胞Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22273: Black PH, Rowe WP. SV-40 induced proliferation of tissue culture cells of rabbit, mouse, and porcine origin. Proc. Soc. Exp. Biol. Med. 114: 721-727, 1963. PubMed: 14120333
