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產(chǎn)品資料

Hs 733.Sk細(xì)胞

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產(chǎn)品名稱: Hs 733.Sk細(xì)胞
產(chǎn)品型號(hào): Hs 733.Sk
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

Hs 733.Sk細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o(wú)菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無(wú)菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來(lái)源和培養(yǎng)基配制是減低污染之*好方法。Hs 733.Sk細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


Hs 733.Sk細(xì)胞  的詳細(xì)介紹

Hs 733.Sk細(xì)胞


是否是腫瘤細(xì)胞: 0

物種來(lái)源: 人

年限: 9 years

生長(zhǎng)狀態(tài): 貼壁生長(zhǎng)

ATCC Number: CRL-7469?

相關(guān)**: 正常

數(shù)量: 大量

細(xì)胞形態(tài): 成纖維樣

運(yùn)輸方式: 凍存運(yùn)輸

器官來(lái)源: 皮膚

Designations: Hs 733.Sk

Hs 733.Sk細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Hs 733.Sk細(xì)胞

Source: Organ: skin

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Cytogenetic Analysis: modal number = 46

Age: 9 years

Gender: male

Ethnicity: Caucasian

Comments: Hs 733.Sk細(xì)胞Patient had IgA deficiency.

Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.

Please see the NBL Repository description.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Hs 733.Sk細(xì)胞culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020


滬公網(wǎng)安備 31011702004356號(hào)

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