VM-2細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： HB-8530?

細(xì)胞形態(tài)： **樣

**類型： IgG1

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

Designations: VM-2

VM-2細(xì)胞Depositors: Stanford University

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human basal cells (skin)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. VM-2細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with human epidermal cells.

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

The antibody binds to a cell surface antigen present on and characteristic of human basal cells and malignant squamous cells.

The antibody does not react with normal human fibroblasts, endothelial or hematopoietic cells.

VM-2細(xì)胞Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 0.45 mM sodium pyruvate and 0.01 M HEPES, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium, 90%; DMSO, 10%

References: 3933: Morhenn V. VM-2細(xì)胞Monoclonal antibody specific for human basal cell surface antigen. US Patent 4,886,745 dated Dec 12 1989