M3/84.6.34細(xì)胞
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
**類型： rat IgG1 kappa
生長狀態(tài)： 懸浮生長
ATCC Number： TIB-168?
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來源： 大鼠
器官來源： 脾
Designations: M3/84.6.34
Depositors: TA Springer
M3/84.6.34細(xì)胞Isotype: rat IgG1 kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against Mac-3 (mouse macrophage antigen, 110000 dalton glycoprotein)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. M3/84.6.34細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: Please acknowledge the origin of this cell line in all relevant publications by citing the following publication(s): [963]
Comments: Animals were immunized with detergent solubilized mouse(C57BL/6) macrophage membrane which had been depleted of previously identified antigens with monoclonal immunoadsorbants.
Like Mac-2, the Mac-3 antigen is not expressed on bone marrow cells (Mac-1 is expressed on bone marrow cells).
Also like Mac-2, Mac-3 appears to be expressed on their monocytic line of differentiation at a stage after divergence from the granulocytic series.
Mac-2 and Mac-3 are present on 69% of macrophages and 0% to 2% of thymocytes.
Expression of Mac-3 is increased during the differentiation from monocyte to activated peritoneal macrophage.
Tested and found negative for ectromelia virus (mousepox).
Propagation: M3/84.6.34細(xì)胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 80%; heat-inactivated fetal bovine serum, 20%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Adherent cells can be dislodged by scraping and cultures established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml.
Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: M3/84.6.34細(xì)胞Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
References: 963: Springer TA. Monoclonal antibody analysis of complex biological systems. Combination of cell hybridization and immunoadsorbents in a novel cascade procedure and its application to the macrophage cell surface. J. Biol. Chem. 256: 3833-3839, 1981. PubMed: 7217058
