H1.6細胞
數(shù)量： 大量
ATCC Number： CRL-2567?
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
細胞形態(tài)： **樣
**類型： IgG1 kappa
生長狀態(tài)： 懸浮生長
年限： *****
器官來源： 脾
Designations: H1.6
Depositors: J Dean
H1.6細胞Isotype: IgG1 kappa
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: spleen
Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid
Cellular Products: immunoglobulin; monoclonal antibody; against human ZP1 glycoprotein
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: ELISA detection zona pellucida glycoprotein 1
Western Blot zona pellucida glycoprotein 1
Age: *****
Comments: H1.6細胞Animals were immunized with recombinant human zonae pellucidae 1 (ZP1).
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
An extracellular matrix that mediates critical steps in fertilization and early development surrounds all vertebrate eggs. In mice and humans, this matrix is known as the zona pellucida and comprises three glycoproteins: ZP1, ZP2 and ZP3.
The H1.6 antibody specifically binds to human ZP1 glycoprotein.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose supplemented with 10 mM HEPES, 0.15 mg/ml oxaloacetate, 0.05 mg/ml pyruvate, 0.0082 mg/ml bovine insulin and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
H1.6細胞Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium H1.6細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
