UV24 (UV sensitive mutant of CHO)細(xì)胞

器官來(lái)源： 卵巢

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 單層、懸浮

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-1866?

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 倉(cāng)鼠

Designations: UV24 (UV sensitive mutant of CHO)

UV24 (UV sensitive mutant of CHO)細(xì)胞Depositors: LH Thompson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: monolayer and suspension

Organism: Cricetulus griseus

Morphology: epithelial-like

Source: Organ: ovary

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Gender: UV24 (UV sensitive mutant of CHO)細(xì)胞female

Comments: This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).

UV24 is a UV sensitive line derived from AA8 (see ATCC CRL-1859).

The line is defective in nucleotide excision repair, is sensitive to bulky adduct mutagens and belongs to excision repair complementation group 2.

Propagation: ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended

Medium Renewal: 2 to 3 times per week

To subculture attached cells, remove the medium and rinse the cells with fresh 0.25% trypsin, 0.03% EDTA.

Let the culture sit at 37C until the cells detach (about 5 minutes).

Add fresh medium, aspirate and transfer to new flasks.

The suspended cells are viable and can be used to start new cultures.

Doubling Time: 13 hrs

References: 1767: Thompson LH, et al.UV24 (UV sensitive mutant of CHO)細(xì)胞 Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene. Somatic Cell Mol. Genet. 10: 183-194, 1984. PubMed: 6584989

1768: Thompson LH, et al. Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 78: 3734-3737, 1981. PubMed: 6943579

1769: Hoy CA, et al. Defective DNA cross-link removal in Chinese hamster cell mutants hypersensitive to bifunctional alkylating agents. Cancer Res. 45: 1737-1743, 1985. PubMed: 3919945

1770: Busch D, et al. Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening. Mutagenesis 4: 349-354, 1989. PubMed: 2687628

32924: Reardon JT, et al. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAKand TFIIH. Proc. Natl. Acad. Sci. USA 93: 6482-6487, 1996. PubMed: 8692841

58395: Thompson LH, et al. Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions. UV24 (UV sensitive mutant of CHO)細(xì)胞Somatic Cell Genet. 8: 759-773, 1982. PubMed: 7163954