HeLa 229細胞
年限： 31 years
細胞形態(tài)： 上皮樣
是否是腫瘤細胞： 0
物種來源： 人
生長狀態(tài)： 貼壁生長
ATCC Number： CCL-2.1?
相關(guān)**： 腺癌
數(shù)量： 大量
運輸方式： 凍存運輸
器官來源： 宮頸
Designations: HeLa 229
HeLa 229細胞Depositors: JT Syverton
Biosafety Level: 2 [Cells contain human papilloma virus (HPV-18) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent (The line can be readily adapted to growth in suspension)
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: cervix
Disease: adenocarcinoma
Cellular Products: keratin
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Virus Susceptibility: Human adenovirus 7
Vesicular stomatitis virus
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes: G6PD, A
Age: HeLa 229細胞31 years
Gender: female
Ethnicity: Black
HeLa Markers: Y
Comments: Relatively resistant to polioviruses.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: HeLa 229細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 23180: Yee C, et al. Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines. Am. J. Pathol. 119: 361-366, 1985. PubMed: 2990217
