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產(chǎn)品資料

MAC 2-48細胞

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產(chǎn)品名稱: MAC 2-48細胞
產(chǎn)品型號: MAC 2-48
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

MAC 2-48細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。MAC 2-48細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


MAC 2-48細胞  的詳細介紹

MAC 2-48細胞

細胞形態(tài): **樣

**類型: IgG1

運輸方式: 凍存運輸

生長狀態(tài): 懸浮生長

ATCC Number: HB-10714?

是否是腫瘤細胞: 0

物種來源: 小鼠

數(shù)量: 大量

Designations: MAC 2-48

MAC 2-48細胞Depositors: P Guyre, PM Morganelli

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: MAC 2-48細胞immunoglobulin; monoclonal antibody

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with human monocytes.

MAC 2-48細胞Spleen cells were fused with NS-1 myeloma cells.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. MAC 2-48細胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

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