COLO 829BL細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

年限： 45 years

器官來源： 外周血

細(xì)胞類型： B**細(xì)胞

ATCC Number： CRL-1980?

是否是腫瘤細(xì)胞： 0

物種來源： 人

細(xì)胞形態(tài)： **樣

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

COLO 829BL細(xì)胞Designations: COLO 829BL

Depositors: GE Moore

Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: peripheral blood

Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. COLO 829BL細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 45 years

Gender: male

Ethnicity: Caucasian

Comments: The COLO 829BL cell line was established at the ATCC by Epstein-Barr virus (EBV) transformation of of a sample of peripheral blood provided by G.E. Moore.

The specimen was taken from a patient prior to chemotherapy for metastatic melanoma.

A cell line was also produced from the melanoma, and is available as COLO 829 (ATCC CRL-1974).

Propagation: COLO 829BL細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2-4 X 10 (5) cells/ml and maintain between 2 X 10 (5) and 2 X 10 (6) cells/ml.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Related Products: COLO 829BL細(xì)胞tumor cell line established from the same patient:ATCC CRL-1974