B72.3細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HB-8108?

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgG1

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

Designations: B72.3

Depositors: NIH

B72.3細(xì)胞Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human mammary tumor cells

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Comments: B72.3細(xì)胞Animals were immunized with membrane enriched fractions of extracts from either of two human breast tumor metastases to the liver (Met 1 and Met 2).

Spleen cells were fused with NS-1 myeloma cells.

The antibody reacts with extracts of Met 1 but not Met 2 or with live BT-20 (ATCC HTB-19), MCF7 (ATCC HTB-22) and ZR-75-1 (ATCC CRL-1500) mammary carcinoma cells or with liver extracts or a variety of live normal and sarcoma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: 3 times per week

B72.3細(xì)胞Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium, 95; DMSO, 5%

References: 1177: Colcher D, et al. A spectrum of monoclonal antibodies reactive with human mammary tumor cells. Proc. Natl. Acad. Sci. USA 78: 3199-3203, 1981. PubMed: 6789331

3537: Colcher D, et al. Process for producing monoclonal antibodies reactive with human breast cancer. B72.3細(xì)胞US Patent 4,522,918 dated Jun 11 1985