MPC-11細(xì)胞
細(xì)胞形態(tài)： **樣
**類型： IgG2b
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
ATCC Number： CCL-167?
相關(guān)**： 多發(fā)性骨髓瘤
品系： BALB/c
細(xì)胞類型： B**細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
數(shù)量： 大量
MPC-11細(xì)胞運(yùn)輸方式： 凍存運(yùn)輸
Designations: MPC-11
Depositors: MD Scharff
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
Source: Strain: BALB/c
Disease: plasmacytoma; myeloma
Cell Type: MPC-11細(xì)胞B lymphocyte;
Cellular Products: immunoglobulin; interleukin-6 (interleukin 6, IL-6)
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Virus Resistance: poliovirus 1
Antigen Expression: H-2d
Comments: The cells contain A type viral particles.
MPC-11 cells have been reported to produce murine IL-6.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. MPC-11細(xì)胞To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 17 hrs
References: 1039: Laskov R, et al. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. II. Assembly of IgG2b immunoglobulin by MPC 11 tumor and culture cells. J. Mol. Biol. 56: 327-339, 1971. PubMed: 5102530
1040: Laskov R, Scharff MD. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits. J. Exp. Med. 131: 515-541, 1970. PubMed: 4189836
1041: Coffino P, et al. Immunoglobulin production: method for quantitatively detecting variant myeloma cells. Science 167: 186-188, 1970. PubMed: 4188146
22823: Blankenstein T, et al. MPC-11細(xì)胞DNA rearrangement and constitutive expression of the interleukin 6 gene in a mouse plasmacytoma. J. Exp. Med. 171: 965-970, 1990. PubMed: 2106569
26031: Potter M, Kuff EL. Disorders in the differentiation of protein secretion in neoplastic plasma cells. J. Mol. Biol. 9: 537-544, 1964. PubMed: 14202284
