CHL/IU [CHL-11]細(xì)胞

ATCC Number： CRL-1935?

器官來源： 肺

生長狀態(tài)： 貼壁生長

年限： newborn

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

細(xì)胞形態(tài)： 成纖維樣

Designations: CHL/IU [CHL-11]

CHL/IU [CHL-11]細(xì)胞Depositors: M Ishidate

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus

Morphology: fibroblast

Source: Organ: lung

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Isolation: CHL/IU [CHL-11]細(xì)胞Isolation date: 1970

Cytogenetic Analysis: modal number = 25

Age: newborn

Gender: female

Comments: This line was derived from the CHL line established by Utakoji in 1970.

The line is useful for screening chemical for ability to induce chromosomal aberrations.

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; bovine calf serum, 10%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: CHL/IU [CHL-11]細(xì)胞A subcultivation ratio of 1:3 to 1:10 is recommended

Medium Renewal: 1 to 2 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 15 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2030

References: 21856: Data book of chromosomal aberration test in vitro. New York: Elsevier; 1988.

22578: Ishidate M Jr., Odashima S. Chromosome tests with 134 compounds on Chinese hamster cells in vitro-- a screening for chemical carcinogens. Mutat. Res. 48: 337-354, 1977. PubMed: 876270

22972: Koyama H, et al. CHL/IU [CHL-11]細(xì)胞A new cell line derived from newborn Chinese hamster lung tissue. Gann 61: 161-167, 1970. PubMed: 5452405