4MBr-5細胞

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

數(shù)量： 大量

細胞形態(tài)： 上皮樣

細胞類型： 上皮細胞

組織來源： bronchus

是否是腫瘤細胞： 0

物種來源： 獼猴

年限： 2 to 3 years

器官來源： 肺

ATCC Number： CCL-208?

4MBr-5細胞相關(guān)**： 正常

Designations: 4MBr-5

Depositors: JL Caputo

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Macaca mulatta

Morphology: epithelial

Source: Organ: lung

Tissue: bronchus

Disease: normal

Cell Type: epithelial

Cellular Products: keratin

Permits/Forms: 4MBr-5細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: May, 1978

Receptors: epidermal growth factor (EGF), expressed

Cytogenetic Analysis: The stemline modal chromosome number is 45 occurring at 68%, and polyploidy at 1.6%. The only marker chromosome 14 pt is found in the majority (60%) of cells. N3 is tetrasomic and N19 is trisomic. There are two copies of X chromosomes, but no Y-like chromosome. The cell line appears to be of female origin.

Age: 2 to 3 years

Comments: This epithelial cell line was derived from the normal bronchial tissue of a 2- to 3-year-old rhesus monkey. The line was initiated in May, 1978 from tissue explants using glass chips. Cells contain PAS-positive cytoplasmic inclusions.

The cells are positive for keratin by immunoperoxidase staining.

Propagation: ATCC complete growth medium: Ham's F-12K Medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 30ng/ml epidermal growth factor and 10% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

4MBr-5細胞Temperature: 37.0°C

Growth Conditions: The line requires epidermal growth factor (EGF) for proliferation.

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: 4MBr-5細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

References: 22657: . . In Vitro 15: 222-223, 1979.

88930: Rubin JS, et al. Purification and characterization of a newly identified growth factor specific for epithelial cells. Proc. Natl. Acad. Sci. USA 86: 802-806, 1989. PubMed: 2915979