CCD-11Lu細胞

細胞類型： 成纖維細胞

是否是腫瘤細胞： 0

物種來源： 人

器官來源： 肺

數量： 大量

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

年限： 9 years

ATCC Number： CCL-202?

相關**： 正常

CCD-11Lu細胞細胞形態(tài)： 成纖維樣

Designations: CCD-11Lu

Depositors: GP Maxwell

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: lung

Disease: normal

Cell Type: CCD-11Lu細胞fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: blood type O; Rh +

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 8,14

D16S539: 9,12

D5S818: 11,13

D7S820: 10,12

THO1: 9

TPOX: 8,11

vWA: 18,20

Cytogenetic Analysis: normal human male; diploid; stable

Age: 9 years

Gender: male

Ethnicity: Asian

Comments: CCD-11Lu細胞The line was derived from normal tissue from patient who died following trauma to the head.

The cells are capable of at least 12 population doublings beyond the current freeze.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin for several minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. CCD-11Lu細胞Subculture every 5 to 7 days.