DS1細(xì)胞

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2484?

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： mouse IgG1 kappa

Designations: DS1

Depositors: DG Sieckmann

DS1細(xì)胞Isotype: mouse IgG1 kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma:lymphoblast B lymphocyte; somatic cell hybrid

Cellular Products: immunoglobulin; monoclonal antibody; against mouse IgMa allotype

Permits/Forms: DS1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: This cell line was deposited at the ATCC by Donna G. Sieckmann, Naval Medical Research Center. The line is distributed for academic research purposes only. The Naval Medical Research Center releases the line subject to the following: 1.) The line or its products must not be distributed to third parties. Commercial interests are the exclusive property of the Naval Medical Research Center. 2.) Any proposed commercial use of the cell line including any use by a for-profit entity must first be negotiated with the Commanding Officer, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500.

Isolation: Isolation date: 1986

Comments: Animals were immunized against TC31, a NMRI strain mouse IgM, kappa monoclonal antibody of the IgMa allotype.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody recognizes the Igh-6.1 allotype on mouse IgM.

It reacts with the IgMa but not the IgMb determinant localized to the CH1 domain of the mu heavy chain. [40006]

DS1 antibody reacts with cells bearing the a,c,f,g,h and j Igh-C haplotypes.

DS1細(xì)胞This antibody can be used as a typing reagent for mouse IgM of the a,c,f,g,h and j haplotypes. It is useful in typing immunoglobulin transgenic mice in which the IgM locus is involved.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 85%; fetal bovine serum, 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 4 to 5 X 10(5) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.

Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: DS1細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 40006: Sieckmann DG, et al. A mouse monoclonal antibody specific for an allotypic determinant of the Igha allele of murine IgM: genetic and functional analysis of Igh-6a epitopes using anti-IgM monoclonal antibodies. Hybridoma 10: 121-135, 1991. PubMed: 1709612

47302: Sieckmann D. A monoclonal mouse antibody that recognizes a mouse IgM allotypic determinant (IgH-6.1). Fed. Proc. 46: 488, 1987.