WR19M.1細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

運輸方式： 凍存運輸

ATCC Number： TIB-70?

相關**： 其他**

品系： BALB/c

Designations: WR19M.1

WR19M.1細胞Depositors: WC Raschke

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology:

Source: Disease: Abelson murine leukemia virus-induced tumor

Strain: BALB/c

Cellular Products: lysozyme; Abelson murine leukemia virus (infectious)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. WR19M.1細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Receptors: complement (C3)

Antigen Expression: H-2d

Gender: female

Comments: This line was derived from the same tumor as WR19L (ATCC TIB-52).

The cells will pinocytose neutral red and phagocytose zymosan and latex beads.

They will also lyse antibody coated sheep erythrocytes.

They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2-).

WR19M.1細胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 1 to 2 times per week

Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells and dispense into new flasks.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum: ATCC 30-2040

References: 1207: Raschke WC, et al. WR19M.1細胞Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198