K6H6/B5細胞

器官來源： **結

是否是腫瘤細胞： 0

物種來源： 人

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

數(shù)量： 大量

ATCC Number： CRL-1823?

相關**： 其他**

細胞形態(tài)： **樣

Designations: K6H6/B5

K6H6/B5細胞Depositors: R Levy

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: human (B cell lymphoma); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: lymph node

Disease: nodular lymphoma

Cell Type: hybridoma: B lymphocyte; somatic cell hybrid

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. K6H6/B5細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma.

The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells.

Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin.

Tested and found negative for ectromelia virus (mousepox).

Comments: The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma. Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: K6H6/B5細胞Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001

Recommended serum: ATCC 30-2020

Cell culture tested DMSO: ATCC 4-X

Erythrosin B vital stain solution: ATCC 30-2404

References: 1625: Carroll WL, et al. K6H6/B5細胞Mouse X human heterohybridomas as fusion partners with human B cell tumors. J. Immunol. Methods 89: 61-72, 1986. PubMed: 3084658