RIN-5F細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2058?

品系： NEDH

組織來源： islet cell tumor

生長狀態(tài)： 貼壁生長

細(xì)胞形態(tài)： 上皮樣

器官來源： 胰腺

數(shù)量： 大量

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

RIN-5F細(xì)胞物種來源： 大鼠

Designations: RIN-5F

Depositors: AF Gazdar, H Oie

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: pancreas

Strain: NEDH

Tissue: islet cell tumor

Cell Type: beta cell;

Cellular Products: insulin; L-dopa-decarboxylase

Permits/Forms: RIN-5F細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Gender: male

Comments: The RIN-5F cell line is a clone derived from the RIN-m rat islet line (ATCC CRL-2057 ). The cells produce and secrete insulin, and produce L-dopa-decarboxylase (a marker for cells having amine precursor uptake and decarboxylation, or APUD, activity). Unlike the parental line they do not produce somatostatin. The parental line was also cloned to establish RIN-14B (ATCC CRL-2059) that produces only somatostatin. These offer models for the study of the biology of pancreatic islet cells, specifically the mechanisms controlling the synthesis, storage and secretion of insulin and somatostatin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Monolayer never becomes confluentVolumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 1. Remove and discard culture medium.2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).4. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.5. RIN-5F細(xì)胞Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting. 6. To remove trypsin-EDTA solution, transfer cell suspension to a centrifuge tube and spin at approximately 125 xg for 5 to10 minutes.7. Discard supernatant and resuspend cells in fresh growth medium8. Add appropriate aliquots of the cell suspension to new culture vessels. 9. Incubate cultures at 37?C. Cells will take about 3 days to attach fully

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Every 3 to 4 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-2057

References: 22483: RIN-5F細(xì)胞Bhathena SJ, et al. Insulin, glucagon, and somatostatin secretion by cultured rat islet cell tumor and its clones. Proc. Soc. Exp. Biol. Med. 175: 35-38, 1984. PubMed: 6141566

22592: Chick WL, et al. A transplantable insulinoma in the rat. Proc. Natl. Acad. Sci. USA 74: 628-632, 1977. PubMed: 191819

22640: Bhathena SJ, et al. Insulin, glucagon, and somatostatin receptors on cultured cells and clones from rat islet cell tumor. Diabetes 31: 521-531, 1982. PubMed: 6295859

23266: Gazdar AF, et al. Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor. Proc. Natl. Acad. Sci. USA 77: 3519-3523, 1980. PubMed: 6106192

23511: Oie HK, et al. Clonal analysis of insulin and somatostatin secretion and L-dopa decarboxylase expression by a rat islet cell tumor. Endocrinology 112: 1070-1075, 1983. PubMed: 6129963