GDM-1細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
器官來(lái)源： 外周血
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 人
運(yùn)輸方式： 凍存運(yùn)輸
年限： 66 - 67 years
ATCC Number： CRL-2627?
數(shù)量： 大量
相關(guān)**： 其他**
細(xì)胞形態(tài)： **樣
GDM-1細(xì)胞Designations: GDM-1
Depositors: H Ben-Bassat
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens deposited as human
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: myelomonoblastic leukemia
Cell Type: monoblast;
Cellular Products: lysozyme [53284]
myeloperoxidase [53284]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. GDM-1細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: complement (C3) [53284]
Fc [53284]
Tumorigenic: No
Antigen Expression: Ia +; myeloid leukemia antigen (M-1) [53284]
Age: 66 - 67 years
Gender: female
Ethnicity: White
Comments: The GDM-1 cell line was established in 1980 from the peripheral blood of a patient with a Philadelphia chromosome negative myeloproliferative disorder, after transformation to acute myelomonoblastic leukemia. [53284]
The cells lack B- and T-cell surface markers including T-associated antigens, E-rosetting capacity, surface and intracytoplasmic immunoglobulins. They are non-specific esterase positive. [53284]
GDM-1細(xì)胞The cells are phagocytic for latex beads and iron particles. Exposure to phorbol 12-myristate 13-acetate (TPA) increases the phagocytic activity. TPA also induces macrophage-like differentiation. [53284]
The cells are negative for Epstein-Barr virus nuclear antigen (EBNA-). [53284]
This cell line is a model system for studying myelomonocytic disorders and leukemias.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 3 X 10 exp5 and 3 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Doubling Time: 24 to 36 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53284: Ben-Bassat H, et al. GDM-1細(xì)胞Establishment and characterization of a new permanent cell line (GDM-1) from a patient with myelomonoblastic leukemia. Leuk. Res. 6: 743-752, 1982. PubMed: 6296552
