Hybridoma 231細(xì)胞
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： **樣
**類型： IgG2a
ATCC Number： HB-9401?
Designations: Hybridoma 231
Depositors: Wistar Institute
Hybridoma 231細(xì)胞Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against canine lymphoma cells
Permits/Forms: Hybridoma 231細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with canine lymphoma cells.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts preferentially with canine monocytes and granulocytes, but not with lymphocytes.
It cross-reacts with human granulocytes.
Propagation: Hybridoma 231細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: fetal bovine serum, 95%; DMSO, 5%
References: 22010: Steplewski Z, Jeglum KA. Hybridoma 231細(xì)胞Monoclonal antibodies against lymphoma-associated antigens, hybrid cell lines producing these antibodies. US Patent 5,169,775 dated Dec 8 1992
