FAK+/+細(xì)胞
器官來源： 胚胎
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
細(xì)胞類型： 成纖維細(xì)胞
ATCC Number： CRL-2645?
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： 成纖維樣
年限： 8 days gestation
Designations: FAK+/+
Depositors: D Ilic
FAK+/+細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology: fibroblast
Source: Organ: embryo
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Oncogene: p53 -
Age: 8 days gestation
Comments: FAK+/+細(xì)胞The FAK+/+ cell line (ATCC CRL-2645) was derived in 1995 from an E8.0 day old mouse embryo that had null mutations only in the p53 gene but not in the FAK gene. Therefore the cells are genetically FAK+/+ p53-/-.
It can be used as a control for the FAK-null (ATCC CRL-2644) cell line.
The FAK-null (ATCC CRL-2644) cell line was derived from an E8.0 day old mouse embryo that had null mutations in both the nonreceptor protein tyrosine kinase FAK (focal adhesion kinase) gene and the p53 gene.
Therefore, the cells are genetically FAK-/- p53-/-.
The embryos from which the cell lines were derived were littermates obtained by crossing FAK+/- p53-/- female and FAK+/- p53-/- male.
Expression of the FAK-related protein-tyrosine kinase, Pyk2, is elevated in FAK-/- p53-/- fibroblasts compared with FAK+/+ p53-/- cells. [53383]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. FAK+/+細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Doubling Time: 12 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 53382: Ilic D, et al. Reduced cell motility and enhanced focal adhesion contact formation in cells from FAK-deficient mice. Nature 377: 539-544, 1995. PubMed: 7566154
53383: Sieg DJ, et al. FAK+/+細(xì)胞Pyk2 and Src-family protein-tyrosine kinases compensate for the loss of FAK in fibronectin-stimulated signaling events but Pyk2 does not fully function to enhance FAK- cell migration. EMBO J. 17: 5933-5947, 1998. PubMed: 9774338
