SV-HUC-1細(xì)胞
細(xì)胞類型： 其他細(xì)胞類型
是否是腫瘤細(xì)胞： 0
物種來源： 人
ATCC Number： CRL-9520?
器官來源： 輸尿管
組織來源： uroepithelium
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
細(xì)胞形態(tài)： 上皮樣
年限： 11 years *****
SV-HUC-1細(xì)胞Designations: SV-HUC-1
Depositors: Wisconsin Alumni Res. Fndn.
Biosafety Level: 2 [CELLS CONTAIN ADENOVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: ureter
Tissue: uroepithelium
Cell Type: SV-HUC-1細(xì)胞epithelial SV40 immortalizedSV40 transformed
Cellular Products: uroepithelial keratins; SV40 T antigen
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
Cytogenetic Analysis: The chromosome number distribution is bimodal with 50% of the cells near diploid (mode = 44 chromosomes) and 50% tetraploid (mode = 88). Both populations consistently showed seven marker chromosomes: 5p+, del(6)(p11), 9q+, 11p+, 15q-, 19p+ and Xp+. Most cells lacked a normal chromosome 15. Cells in the tetraploid population did not exactly duplicate the diploid population in that most had a 14q/21 translocation involving two chromosomes 14 and two chromosomes 21, while the diploid cells had normal chromosomes 14 and 21 and a 6q/14q translocation.
Age: 11 years *****
Gender: male
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% Trypsin, 0.03% EDTA solution and allow the flask to sit at room temperature for 20 minutes. SV-HUC-1細(xì)胞Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: 1:2 to 1:5
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
derivative:ATCC CRL-9519
References: 3992: Reznikoff CA, Christian BJ. SV-HUC-1細(xì)胞Human uroepithelial cell. US Patent 4,980,290 dated Dec 25 1990
