5c8細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

ATCC Number： HB-10916?

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

細(xì)胞形態(tài)： **樣

**類型： mouse IgG2a

運(yùn)輸方式： 凍存運(yùn)輸

Designations: 5c8

Depositors: Trustees of Columbia University

Isotype: mouse IgG2a

5c8細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against T-B activating molecule T-BAM, ligand for CD40 (CD40L, CD154)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with D1.1 cells (see ATCC CRL-10915).

Spleen cells were fused with Sp2/0 myeloma cells.

5c8細(xì)胞The antibody recognizes an antigen present on T cells that is important to the T cell mediated activation of B cells.

The antigen is a ligand for the CD40 molecule on the B cell surface.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml.

Interval: Maintain between 1 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: 5c8細(xì)胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22085: Lederman S, et al. Murine monoclonal antibody (5c8) recognizes a human glycoprotein on the surface of T-lymphocytes, compositions containing same. US Patent 5,474,771 dated Dec 12 1995

72024: Lederman S, et al. Methods for treating autoimmune diseases with 5C8-specific antibodies. US Patent 6,592,868 dated Jul 15 2003

90139: .5c8細(xì)胞 Protein recognized by an antibody that specifically binds an epitope that is specifically bound by monoclonal antibody 5c8. U.S. Patent 6,793,924 dated Sep 21 2004