9L/lacZ細胞
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
器官來源： 大腦
ATCC Number： CRL-2200?
相關**： 其他**
數(shù)量： 大量
細胞類型： 其他細胞類型
細胞形態(tài)： 成纖維樣
是否是腫瘤細胞： 0
物種來源： 大鼠
Designations: 9L/lacZ
Depositors: LA Lampson
9L/lacZ細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: fibroblast
Source: Organ: brain
Disease: gliosarcoma
Cell Type: glial cell;
Cellular Products: beta galactosidase (beta-gal)
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Tumorigenic: 9L/lacZ細胞Yes
Comments: The 9L/lacZ cell line was developed in 1989 from the 9L cell line (rat nitrosourea induced gliosarcoma cell line).
9L cells were infected with the BAG replication deficient retroviral vector carrying the E. coli lacZ gene encoding beta-gal and the Tn5 neomycin gene, which confers resistance to G418.
The cells were cultured in G418 for 14 days, cloned, and evaluated for beta-gal production.
9L/lacZ produced high levels of the enzyme, and was selected for study.
The cells constitutively express the lacZ reporter gene product, E. coli derived beta-gal, as revealed on tissue sections by histochemical stain, and single tumor cells can be identified.
Lymphocytes and other responding cells can be identified by double labeling with antibodies on the same slide.
The contrast between stained cells and background facilitates image analysis.
This is one of few models that permit quantitative analysis of microscopic tumor in the brain.
The tumor mimics important features of human brain tumor growth and spread.
The beta-gal expression is very stable, but cells may need to be re-cloned after months of growth in culture.
Propagation: 9L/lacZ細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, and 1 mM sodium pyruvate 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, rinse with PBS and add fresh 0.25% trypsin, 0.03% EDTA solution and incubate at room temperature (or 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22204: Lampson LA, et al. Interactions between leucocytes and individual brain tumor cells in the rat brain. J. Neuro-Oncol. 7: S17, 1990.
22438: Lampson LA, et al. Exploiting the lacZ reporter gene for quantitative analysis of disseminated tumor growth within the brain: use of the lacZ gene product as a tumor antigen, for evaluation of antigenic modulation, and to facilitate image analysis of tumor growth in situ. Cancer Res. 53: 176-182, 1993. PubMed: 8416743
23091: Lampson LA, et al. 9L/lacZ細胞Disseminating tumor cells and their interactions with leukocytes visualized in the brain. Cancer Res. 52: 1018-1025, 1992. PubMed: 1737331
90374: Dutta T, et al. Robust ability of IFN-gamma to upregulate class II MHC antigen expression in tumor bearing rat brains. J. Neuro-Oncol. 64: 31-44, 2003. PubMed: 12952284
