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產(chǎn)品資料

RPMI 7666細胞

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產(chǎn)品名稱: RPMI 7666細胞
產(chǎn)品型號: RPMI 7666
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

RPMI 7666細胞應如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。RPMI 7666細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


RPMI 7666細胞  的詳細介紹

RPMI 7666細胞


年限: 32 years

ATCC Number: CCL-114?

生長狀態(tài): 懸浮生長

數(shù)量: 大量

器官來源: 外周血

運輸方式: 凍存運輸

細胞形態(tài): **樣

細胞類型: B**細胞

是否是腫瘤細胞: 0

物種來源: 人

Designations: RPMI 7666 [Gerner 7666]

Depositors: GE Moore

RPMI 7666細胞Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast


Source: Organ: peripheral blood

Cell Type: B lymphoblast;

Cellular Products: immunoglobulin; Epstein-Barr virus (EBV)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits.RPMI 7666細胞 Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: HLA A3, Aw29, B7, B12; Blood type B+

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 8,12

D16S539: 9,11

D5S818: 11,12

D7S820: 8,11

THO1: 7,9.3

TPOX: 8,11

vWA: 14,16

Age: 32 years

Gender: male

RPMI 7666細胞Ethnicity: Caucasian

Comments: Biological Safety CLASS II.

EBNA positive.

The cells produce Epstein-Barr virus.

Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Cultures can be maintained by addition or replacement of medium. Establish new cultures at 5 X 10 exp5 viable cells/ml.

References: 22312: Moore GE, et al. Culture of normal human leukocytes. RPMI 7666細胞JAMA 199: 519-524, 1967. PubMed: 4960081


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