QNR/D細(xì)胞

細(xì)胞類型： 其他細(xì)胞類型

組織來源： neuroretina

是否是腫瘤細(xì)胞： 0

物種來源： 其他

生長狀態(tài)： 貼壁生長

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： CRL-2532?

年限： embryo, 7 days gestation

Designations: QNR/D

QNR/D細(xì)胞Depositors: B Pessac, D Trisler

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Coturnix coturnix japonica

Morphology:

Source: Tissue: neuroretina

Cell Type: neuronalinfected with Rous sarcoma virus mutant ts NY-68

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QNR/D細(xì)胞Age: embryo, 7 days gestation

Comments: Neuroretinas were dissected from normal quail embryos, dissociated and immortalized by infection with Rous sarcoma virus (RSV) mutant ts NY-68 to establish the QNR ts NY-68 mixed cell line. [48961]

QNR ts NY-68 was subsequently cloned to establish the QNR/D cell line. The cells are routinely maintained at 38.5 to 39C. The permissive temperature for transformation is 36C. [48961]

Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors; glial (Muller) cells; horizontal, bipolar, amacrine; and ganglion neuronal cells. [48961]

The cell line displays properties of amacrine and/or ganglion cells.

QNR/D cells can generate tetrodotoxin(TTX)-inhibitable action potentials on electrical stimulation. They have high glutamic acid decarboxylase (GAD) activity and they bind monoclonal antibodies raised against chick embryo neuroretina. [48961]

QNR/D cells (ATCC CRL-2532) and QNR/K2 cell (ATCC CRL-2533) were transplanted into chicken embryo eyes. Implanted QNR/D cells migrate only to the retinal ganglion and amacrine cell layers and project neurites in the plane of retina. [48964]

In contrast, QNR/K2 cells migrate through the ganglion and amacrine layers, locate in the inner nuclear layer, and project processes across the retina. [48964]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. QNR/D細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Max Temperature: 39.0°C

Min Temperature: 38.5°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 38.5?C.to 39?C

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: QNR/D細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 48961: Pessac B, et al. A neuronal clone derived from a Rous sarcoma virus-transformed quail embryo neuroretina established culture. Nature 302: 616-618, 1983. PubMed: 6300691

48964: Trisler D, et al. Retinal engineering: engrafted neural cell lines locate in appropriate layers. Proc. Natl. Acad. Sci. USA 93: 6269-6274, 1996. PubMed: 8692804

51410: Cohen-Salmon M, et al. Characterization of the promoter of the human KAL gene, responsible for the X-chromosome-linked Kallmann syndrome. Gene 164: 235-242, 1995. PubMed: 7590336