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產(chǎn)品資料

3C11細胞

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產(chǎn)品名稱: 3C11細胞
產(chǎn)品型號: 3C11
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

3C11細胞應(yīng)如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。3C11細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


3C11細胞  的詳細介紹

3C11細胞

生長狀態(tài): 懸浮生長

是否是腫瘤細胞: 0

物種來源: 小鼠

數(shù)量: 大量

ATCC Number: HB-8511?

運輸方式: 凍存運輸

細胞形態(tài): **樣

**類型: IgG1; kappa light chain

Designations: 3C11

3C11細胞Depositors: Temple University

Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:3C11細胞 immunoglobulin; monoclonal antibody; against bacterial cell wall peptidoglycan

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with Streptococcus mutans (BHT strain) bacterial cell wall peptidoglycan.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with cell wall peptidoglycans from a variety of gram- and gram+ organisms.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. 3C11細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 3549: Jackson DE, et al. 3C11細胞Monoclonal antibodies to peptidoglycan and methods of preparing same. US Patent 4,596,769 dated Jun 24 1986

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