Rn1T細(xì)胞
運(yùn)輸方式： 瓶裝運(yùn)輸
年限： 2 years
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 褐鼠
數(shù)量： 大量
器官來(lái)源： 乳腺
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
ATCC Number： CRL-6598?
Designations: Rn1T
Rn1T細(xì)胞Biosafety Level: 1
Shipped: flask
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus
Morphology:
Source: Organ: mammary gland
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 2 years
Gender: female
Comments: Rn1T細(xì)胞Spontaneous tumors in a pet rat.
Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Note: This item is distributed only within the 50 United States. It is not available for international distribution.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Rn1T細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium Rn1T細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
