3B18細(xì)胞

生長狀態(tài)： 混合型生長

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： HB-8654?

細(xì)胞形態(tài)： **樣

**類型： IgG1

Designations: 3B18

3B18細(xì)胞Depositors: Salk Institute for Biological Studies

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed: adherent and suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Cellular Products: immunoglobulin; monoclonal antibody; against a human mammary tumor cell cytoplasmic antigen

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Comments: Animals were immunized with MCF7 cells (ATCC HTB-22).

Spleen cells were fused with PAI myeloma cells.

The antibody cross-reacted with some cases of fibrocystic mammary disease and one of two colon carcinomas tested.

It did not react with normal tissue nor with 17 of 18 non-mammary malignancies tested.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 0.1 mM hypoxanthine, 0.02 mM thymidine, 0.01 mM 2-mercaptoethanol, and non-essential amino acids, 90%; horse serum, 10%

3B18細(xì)胞Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by transferring floating cells to additional flasks.

Attached cells may be subcultured by scraping.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

References: 3907: Dulbecco R, et al. 3B18細(xì)胞Monoclonal antibody specific for a mammary tumor cytoplasmic antigen. US Patent 4,628,032 dated Dec 9 1986