SJK-132-20細(xì)胞
ATCC Number： CRL-1640?
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： **樣
**類型： IgG1
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 懸浮生長
數(shù)量： 大量
Designations: SJK-132-20
Depositors: D Korn
Isotype: IgG1
SJK-132-20細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human DNA polymerase alpha
Permits/Forms: SJK-132-20細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: H-2db
Comments: Mice were immunized with fraction VIII human DNA polymerase alpha.
Alpha.
Spleen cells were fused with NS-1 myeloma cells.
The antibody neutralizes polymerase alpha activity, and reacts with polymerases from several species.
The cells need to be in culture for 6 to 7 days before antibody production is detectable.
Tested and found negative for ectromelia virus (mousepox).
Formerly ATCC TIB-177.
Propagation: SJK-132-20細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.
References: 959: Hu SZ, et al. Preparation and preliminary characterization of monoclonal antibodies against human DNA polymerase alpha. J. Biol. Chem. 257: 8386-8390, 1982. SJK-132-20細(xì)胞PubMed: 7085672
