33D1細胞
運輸方式： 凍存運輸
細胞形態(tài)： **樣
**類型： IgG2b
數(shù)量： 大量
ATCC Number： TIB-227?
是否是腫瘤細胞： 0
物種來源： 大鼠
生長狀態(tài)： 懸浮生長
Designations: 33D1
33D1細胞Depositors: RM Steinman
Isotype: IgG2b
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products:33D1細胞 immunoglobulin; monoclonal antibody; against mouse dendritic cells (cytotoxic)
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Comments: Animals were immunized with mouse spleen and lymph node dendritic cells.
Spleen cells were fused with P3X63Ag8-U1 myeloma cells.
The antigen recognized by the 33D1 antibody appears to be present only on dendritic cells (10000 to 15000 sites/cell).
The antigen does not appear to be immune response associated antigen or Ia antigen.
Tested and found negative for ectromelia virus (mousepox).
Propagation: 33D1細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1663: Nussenzweig MC, et al. A monoclonal antibody specific for mouse dendritic cells. Proc. Natl. Acad. Sci. USA 79: 161-165, 1982. PubMed: 6948298
1664: Steinman RM, et al. 33D1細胞Dendritic cells are the principal stimulators of the primary mixed leukocyte reaction in mice. J. Exp. Med. 157: 613-627, 1983. PubMed: 6185614
