Sf 1 Ep (NBL-11)細(xì)胞

ATCC Number： CCL-68?

運輸方式： 凍存運輸

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

年限： *****

是否是腫瘤細(xì)胞： 0

物種來源： 其他

器官來源： 皮膚

細(xì)胞形態(tài)： 上皮樣

Sf 1 Ep (NBL-11)細(xì)胞組織來源： epidermis

Designations: Sf 1 Ep (NBL-11)

Depositors: AJ Kniazeff, WA Nelson-Rees, RB Owens

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Sylvilagus floridanus

Morphology: epithelial

Sf 1 Ep (NBL-11)細(xì)胞Source: Organ: skin

Tissue: epidermis

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Resistance: poliovirus 2; coxsackievirus A9, B5; adenovirus 5

Age: *****

Gender: male

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: Sf 1 Ep (NBL-11)細(xì)胞37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Twice per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium Sf 1 Ep (NBL-11)細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020