Schneider's Drosophila Line 2細(xì)胞

器官來源： 胚胎

年限： embryo; 20 to 24 hours

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-1963?

是否是腫瘤細(xì)胞： 0

物種來源： 果蠅

Schneider's Drosophila Line 2細(xì)胞細(xì)胞形態(tài)： 上皮樣

Designations: Schneider's Drosophila Line 2 [D. Mel. (2), SL2]

Depositors: IR Schneider

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension or loosely adherent

Organism: Drosophila melanogaster

Morphology: epithelial

Source: Organ: embryo

Permits/Forms: Schneider's Drosophila Line 2細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1969

Applications: transfection host (Roche Transfection Reagents)

Age: embryo; 20 to 24 hours

Comments: The cells have been shown to support the growth of the insect stages of malaria parasites [Pubmed ID: 1734521].

Propagation: ATCC complete growth medium: Schneider's Drosophila Medium, 90%; heat-inactivated fetal bovine serum, 10%. NOTE: the fetal bovine serum should be selected for ability to support the growth of insect cell lines.

This medium is formulated for use without CO2.

Temperature: Schneider's Drosophila Line 2細(xì)胞23.0°C; (Max. 24.0 °C, Min. 22.0°C)

Note: The cells will grow as a loose monolayer (or in suspension) at any temperature between 16.0°C and 28.0°C.

Subculturing: Protocol: Gently resuspend the cells by pipetting medium across the monolayer or (for larger flasks) by slapping the flask against the base of the palm. Dilute the cell suspension to the desired concentration with fresh medium.

Maintain cultures at a cell concentration between 5 X 10(4) and 4 X 10(5) cells/cm2.

Subcultivation Ratio: A subcultivation ratio of 1:10 or greater is recommended

Medium Renewal: At the time of subcultivation

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: approximately 67 hours

References: 22960: Warburg A, Miller LH. Schneider's Drosophila Line 2細(xì)胞Sporogonic development of a malaria parasite in vitro. Science 255: 448-450, 1992. PubMed: 1734521

22983: Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morphol. 27: 353-365, 1972. PubMed: 4625067