ED1-19-1-6-5細(xì)胞
運輸方式： 凍存運輸
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
細(xì)胞形態(tài)： **樣
**類型： IgG2a
是否是腫瘤細(xì)胞： 0
物種來源： 大鼠
ATCC Number： HB-90?
ED1-19-1-6-5細(xì)胞Designations: ED1-19-1-6-5
Depositors: JF Kearney
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse IgG2
Permits/Forms: ED1-19-1-6-5細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with purified mouse IgG2b (lambda).
Lymph node cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with both IgG2a and IgG2b.
Tested and found negative for ectromelia virus (mousepox).
A feeder layer of mouse peritoneal macrophages or X-irradiated STO cells (ATCC 56-X) is necessary for successful initiation of a culture from a thawed ampule.
Propagation: ED1-19-1-6-5細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 10(5) viable cells/ml into new culture vessels pre-plated with ATCC 56-X feeder layer (irradiated STO cells). Maintain between 1 X 10 (5) and 1 X 10 (6) cells/ml.
Related Products: feeder layer cells:ED1-19-1-6-5細(xì)胞ATCC 56-X
